human melanoma cell lines a375 (ATCC)

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ATCC human melanoma cell lines a375
Human Melanoma Cell Lines A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a375+melanoma/A-375/pm42883943-178-1-6
Average 99 stars, based on 5311 article reviews

human melanoma cell lines a375 - by Bioz Stars, 2026-11

99/100 stars

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Article Title: RNA splicing dynamics in CD8 T cells uncovers isoforms that impact T cell-mediated cancer immunotherapy
Article Snippet: .. Cell line sources were as follows: Jurkat E6-1 (ATCC, TIB-152), HEK293 cells (CRL-1573), T2 (ATCC, CRL-1992), T2-RFP (generated with Incucyte® Nuclight NIR Lentivirus, Sartorius), A375 melanoma (ATCC, CRL-1619), A375-luc (a kind gift from Polina Stepensky). .. Jurkat, T2 and A375 cells were maintained in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine (Gibco).

Cell Culture:

Article Title: Design, synthesis and biological evaluation of new ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) derivatives as potential antitumor agents.
Article Snippet: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) is a naturally occurring ent-kaurane diterpenoid isolated from the traditional Chinese herbal medicine Pteris semipinnata L., a fern plant known for its reported antitumor properties.. In an effort to expand the pool of natural scaffold-based compounds for anticancer purposes, novel derivatives of 5F have been synthesized by modifying functional groups at C-11 and C-19.. These derivatives have been evaluated for their anti-proliferative activities against a panel of cancer cell lines.

Article Title: Antigen-scaffolds drive preferential expansion of functional genetically engineered CAR and TCR T cells
Article Snippet: Cells were counted and activated on 5 μg/mL plate-bound anti-CD3 (clone: UCHT1, Biolegend) with 2 μg/mL soluble anti-CD28 (clone: 28.2, Biolegend) in X-vivo-15 (Lonza, BE02-060Q) + 5 % human serum (Sigma Aldrich Heat Inactivated H3667), supplemented with 200 IU/mL recombinant human IL2 (Peprotech), 5 ng/ml IL7 (Peprotech), and 5 ng/ml IL15 (Peprotech). .. The human cell lines Jeko-1 (B cell lymphoma) and A375 (melanoma) were obtained from American Type Culture Collection (ATCC) and cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. .. For cytotoxicity assay and in vivo tumour models, these cell lines were transduced with mCherry-Luc lentiviral vector (Supplementary Table 1) and were sorted on a FACSmelody (BD Biosciences) to establish new cultures.

Multiple Displacement Amplification:

Article Title: Ole-4, a novel synthetic derivative of Oleuropein, enhances antitumor immunity and modulates kinase activity-dependent signaling.
Article Snippet: .. AR TIC LE IN PR ES S Cell lines and cell cultures The human cancer cell lines SKBR3, MCF7, MDA-MB231 (breast), SKOV3 (ovary), HeLa (cervix), A375 (melanoma), HCT116 (colon), the murine cancer cell lines B16.F1 (melanoma), CT26 (colon), YAC-1 (lymphoma) and WEHI-164 (fibrosarcoma) were purchased from ATCC. ..

DNA Profiling:

Article Title: Heterogeneity of glucose metabolism and uptake identifies distinct cancer cell and cancer stem cell phenotypes
Article Snippet: .. We used three human cancer cell lines that represent diverse common human malignancies; A375 (melanoma), FaDu (squamous cell carcinoma of head and neck), and MCF7 (estrogen receptor positive luminal breast cancer), all obtained from the American Type Culture Collection (Manassas, VA, USA) and authenticated by STR DNA profiling (Eurofins Genomics, Ebersberg, Germany). .. Unless stated otherwise, cells were cultured in high glucose Dulbecco’s modified Eagle medium (DMEM; D5648 Sigma Aldrich, St Louis, MO, USA) supplemented with 100 U/ml penicillin/100 μg/ml streptomycin (Biosera XC-A4122/100; BioTech a.s. Prague, Czech Republic), 1% sodium pyruvate, and 10% heat-inactivated fetal bovine serum (Biosera S-FBS-SA-015).

Planar Chromatography:

Article Title: Demonstration of SLU7 as a new pan-cancer target
Article Snippet: .. Human cell lines PLC/PRF/5 (HCC), A375 (melanoma), HCT116 (colon cancer), A549 (NSCLC), HUCCT-1 (iCCA), TFK-1 (eCCA), mouse cell lines Hepa 1.6 (HCC), B16/F10 (melanoma), and 4T1 (breast cancer) were obtained from the American Type Culture Collection (ATCC). .. All cell lines were authenticated by short tandem repeat (STR) profiling and routinely tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07-318).

Article Title: Demonstration of SLU7 as a new cancer target.
Article Snippet: .. Human cell lines PLC/PRF/5 (HCC), A375 (melanoma), HCT116 (colon cancer), A549 (NSCLC), HUCCT-1 (iCCA), TFK-1 (eCCA), mouse cell lines Hepa 1.6 (HCC), B16/F10 (melanoma), and 4T1 (breast cancer) were obtained from the American Type Culture Collection (ATCC). .. All cell lines were authenticated by short tandem repeat (STR) profiling and routinely tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, #LT07–318).

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